Research Faculty #17

General Information

Academic History

Canada Research Chair in Plant Cell Biology and Professor; 
B.Sc. Biology (1983), Carleton; 
Ph.D. Plant Cell Biology (1989) Australian National Univ (ANU); 
National Research Fellow (1988-91) ANU; 
Humboldt Fellow (1992-1993), Univ. Heidelberg; 
Queen Elizabeth II Fellow (1992-1998), ANU; 
Fellow/Senior Fellow (1996-2004), ANU.

Contact Information

Office Phone
Office Location
Office Room 2325/Lab 2322 & 2312 Biological Sciences Bldg
Lab Phone

Research Information

Research Abstract

Plant Cell Biology and Molecular Genetics; organization of the cytoskeleton and its role in cell wall formation, intracellular motility and growth anisotropy in the higher plant Arabidopsis thaliana and the characean algae; plant responses to abiotic and endogenous signals.

Research Interests

1) The control of microtubule dynamics by microtubule-associated proteins.

2) Microtubule polymer activities and the spatial organization of cortical microtubule arrays.

3) The function of cortical microtubule arrays in the mechanical properties of cellulosic cell walls.

Team Members

Chris Ambrose (Post-doctoral Fellow)
Amanda Catching (Research Assistant)
Caitlin Donnelly (MSc candidate)
Ryan Eng (MSc candidate)
Miki Fujita (Post-doctoral Fellow) 
Sylwia Wojas Jancowski (Post-doctoral Fellow)
Eric Johnson (PhD candidate) 
Bettina Lechner (Post-Doctoral Fellow; DFG Fellow)
Yuan Ruan (PhD candidate)
Laura Tamblyn (Honours Student) 
Daniel Zhao (MSc Candidate)

Selected Publications

Galway ME, Eng RC, Schiefelbein JW, Wasteneys GO (2011) Root hair-specific disruption of cellulose and xyloglucan in AtCSLD3 mutants, and factors affecting the post-rupture resumption of mutant root hair growth.Planta. Published Online 29 January 2011,DOI 10.1007/s00425-011-1355-6

Allard JF, Ambrose JC, Wasteneys GO, Cytrynbaum EN. (2010) A mechanochemical model explains interactions between cortical microtubules in plants. (2010) Biophysical Journal 99, 1082-1090

Allard JF, Wasteneys GO, Cytrynbaum EN. (2010) Mechanisms of self-organization of cortical microtubules in plants revealed by computational simulations. Mol. Biol. Cell 21, 278-286

Foissner I, Menzel D, Wasteneys GO (2009) Microtubule-dependent motility and orientation of the cortical endoplasmic reticulum in elongating characean internodal cells. Cell Motil. Cytoskeleton 66: 142-155. 

Wasteneys GO and Ambrose JC (2009) Spatial Organization of Plant Cortical Microtubules: Close Encounters of the 2-D Kind. Trends in Cell Biology, 19: 62-71. .

Walia, A, Lee, J.S., Wasteneys, G., and Ellis, B. (2009). Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells. Plant J 59, 565-575. Link.

Holzinger, A., Kawamura, E., and Wasteneys, G.O. (2009). Strategies for imaging microtubules in plant cells. Methods Mol Biol 586, 243-262.

Kawamura E, Wasteneys GO (2008) MOR1, the MAP215 homologue of Arabidopsis thaliana, promotes rapid growth and shrinkage and suppresses the pausing of microtubules in vivo. J Cell Sci 121: 4114-4123. 

Ambrose, J.C., Wasteneys, G.O. (2008). CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules. Mol Biol Cell. 19: 4730-4737 

McFarlane HE, Young RE, Wasteneys GO, Samuels AL. (2008) Cortical microtubules mark the mucilage secretion domain of the plasma membrane in Arabidopsis see coat cells. Planta 207: 1363-1375. Link

Sakai, T., Honing, H., Nishioka, M., Uehara, Y., Takahashi, M., Fujisawa, N., Saji, K., Seki, M., Shinozaki, K., Jones, M.A., Smirnoff, N., Okada, K., Wasteneys, G.O. (2008). Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal-cell morphogenesis in Arabidopsis. Plant J. 53, 157-171. 

Wasteneys GO and Collings DA (2007) The Cytoskeleton and Co-ordination of Directional Expansion in a Multicellular Context. In: The Expanding Cell. Plant Cell Monogr (5) J.-P. Verbelen, K Vissenberg (Eds) Springer-Verlag Berlin Heidelberg, pp 217-248.

Ambrose, J.C., Shoji, T., Kotzer, A.M., Pighin, J.A., Wasteneys, G.O. (2007). The Arabidopsis CLASP gene encodes a microtubule-associated protein involved in cell expansion and division. Plant Cell 19, 2763-2775. 

Foissner, I., Wasteneys, G.O. (2007). Wide-ranging effects of eight cytochalasins and latrunculin A and B on intracellular motility and actin filament reorganization in characean internodal cells. Plant Cell Physiol 48, 585-597.

Holzinger A, Wasteneys GO, Lütz C (2007) Investigating Cytoskeletal Function in Chloroplast Protrusion Formation in the Arctic- Alpine Plant Oxyria digyna. Plant Biology 9: 400-410.

Talbot MJ, Wasteneys GO, MCCurdy DW, Offler CE (2007) Deposition patterns of cellulose microfibrils in flange wall ingrowths of transfer cells indicate clear parallels with those of secondary wall thickenings Functional Plant Biol34, 307–313. Link

Talbot MJ, Wasteneys GO, Offler CE, MCCurdy DW (2007) Cellulose Synthesis is Required for Deposition of Reticulate Wall Ingrowths in Transfer Cells. Plant Cell Physiol 48: 147-158. Link

Kawamura, E., Himmelspach, R., Rashbrooke, M.C., Whittington, A.T., Gale, K.R., Collings, D.A., Wasteneys, G.O. (2006). MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root. Plant Physiol 140, 102-114. 

Collings, D.A., Lill, A.W., Himmelspach, R., Wasteneys, G.O. (2006). Hypersensitivity to cytoskeletal antagonists demonstrates microtubule-microfilament cross-talk in the control of root elongation in Arabidopsis thaliana. New Phytol 170, 275-290. 

Wasteneys, G.O., Fujita, M. (2006). Establishing and maintaining axial growth: wall mechanical properties and the cytoskeleton. J Plant Res 119, 5-10. 

Kotzer, A.M., Wasteneys, G.O. (2006). Mechanisms behind the puzzle: microtubule-microfilament cross-talk in pavement cell formation. Can J Bot 84, 594-603. Link

Collings DA, Wasteneys GO. (2005) Actin Microfilament and Microtubule Distribution Patterns in the Expanding Root of Arabidopsis thaliana. Can J Bot 83: 579-590. Link

Roudier F, Fernandez AG, Fujita M, Himmelspach R, Borner, GH, Schindelman G, Song S, Baskin TI, Dupree, P, Wasteneys GO, Benfey PN (2005) COBRA, an Arabidopsis extracellular glycosylphosphatidylinositol-anchored protein, specifically controls highly anisotropic expansion through its involvement in cellulose microfibril orientation.Plant Cell 17: 1749-1763. Link

Fu Y, Gu Y, Zheng Z, Wasteneys GO, Yang Z. (2005) Arabidopsis interdigitating cell growth requires two antagonistic pathways with opposing effects on cell morphogenesis. Cell 120: 687-700. Link

Kazama H, Dan H, Imaseki H, Wasteneys GO (2004) Transient exposure to ethylene stimulates cell division and alters the fate and polarity of hypocotyl epidermal cells. Plant Physiol. 134: 1614-1623. Link

Wasteneys GO, Yang Z (2004) The cytoskeleton becomes multidisciplinary. Plant Physiol. 136, 3853-3854. Link1,Link2

Wasteneys GO, Yang Z (2004) New views on the Plant Cytoskeleton. Plant Physiol 136:3884-3891. Link

Wasteneys GO (2004) Progress in understanding the role of microtubules in plant cells. Curr. Opin Plant Biology, Vol 7, Issue 6: 651-660. Link

Himmelspach R, Williamson RE, Wasteneys GO. (2003) Cellulose microfibril alignment recovers from DCB-induced disruption despite microtubule disorganization. Plant J. 36: 565-575. Link

Sugimoto K, Himmelspach R, Williamson RE, Wasteneys GO. (2003). Mutation or drug-dependent microtubule disruption causes radial swelling without altering parallel cellulose microfibril deposition in Arabidopsis root cells. Plant Cell, 15: 1414-1429. Link

Buer CS, Wasteneys GO, Masle J. (2003) Ethylene modulates root-wave responses in arabidopsis. Plant Physiol.132: 1085-1096. Link

Dan H, Imaseki H, Wasteneys GO, Kazama H (2003) Ethylene stimulates endoreduplication but inhibits cytokinesis in cucumber hypocotyl epidermis. Plant Physiol. 133: 1726-1731. Link

Wasteneys GO, Galway ME. (2003) Remodelling the Cytoskeleton for Growth and Form: An overview with some new views. Ann Rev Plant Biol. 54: 691-722. Link

Wasteneys GO (2003) Microtubules show their sensitive nature. Plant Cell Physiol. 44, 653-654. Link

Selected earlier publications:

Wasteneys GO. (2002) Microtubule organization in the green kingdom: chaos or self-order? J Cell Sci 115: 1345-1354. Link

Whittington AT, Vugrek O, Wei KJ, Hasenbein NG, Sugimoto K, Rashbrooke MC, Wasteneys GO. (2001) MOR1 is essential for organizing cortical microtubules in plants. Nature 411: 610-613. Link

Book chapters:

Holzinger, A., Kawamura, E., and Wasteneys, G.O. (2009). Strategies for imaging microtubules in plant cells. Methods Mol Biol 586, 243-262.

Wasteneys GO, Collings DAC (2007) The Cytoskeleton and Co-Ordination of Directional Expansion in a Multicellular Context. Plant Cell Monogr Springer_Verlag Berlin Heidelberg DOI 10.1007/7089_2006_077/Published online: October 2006

Wasteneys GO, Collings DAC (2004) Expanding beyond the great divide: the cytoskeleton and axial growth. In: The Plant Cytoskeleton in Cell Differentiation and Development. Annual Plant Reviews, Volume 10, pp. 83-115, Blackwell Publishing. Ed. Hussey PJ.